Moleküler Biyoloji ve Genetik Bölümü / Department of Molecular Biology and Genetics
https://hdl.handle.net/20.500.12294/215
Moleküler Biyoloji ve Genetik Bölümüne ait koleksiyonlar bu alt bölümde listelenir.
2024-03-28T09:22:16Z
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Marine Streptomyces sp. PGC 39
https://hdl.handle.net/20.500.12294/4076
Marine Streptomyces sp. PGC 39
Ahmad, Rabbia; Tousif, Muhammad Imran; Nazir, Mamona; Yaqoob, Asma; Shah, Syed Adnan Ali; Zengin, Gokhan; Uba, Abdullahi Ibrahim; Muhammad, Shabbir
Macrolidycin (1) and pyrachlomycin (2) were isolated from the culture broth of the marine bacterium Streptomyces sp. GSCWU 39. The isolates were characterized due to 1D and 2D-NMR spectral analyses and high resolution mass spectrometry, and were evaluated for their anti-bacterial and anti-fungal activities. At a concentration of 30 μg/mL, the compounds 1 and 2 inhibited the growth of a gram positive bacterium Streptomyces viridochromogenes and the fungus Mucor miehei. Further in order to deep the understanding for antibacterial and antifungal activities the isolates were docked against methyltransferase AviRb and ergosterol. Compounds 1 and 2 had strong binding energies of −7.32 and −6.32 kcal/mol, respectively, for the methyltransferase AviRb. Additionally, our findings for antifungal activities are further corroborated by the docking results of ergosterol with compounds 1 and 2. © 2024 Elsevier Ltd
2024-01-01T00:00:00Z
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Chemical exploration of different extracts from Phytolacca americana leaves and their potential utilization for global health problems: ın silico and network pharmacology validation
https://hdl.handle.net/20.500.12294/4069
Chemical exploration of different extracts from Phytolacca americana leaves and their potential utilization for global health problems: ın silico and network pharmacology validation
Uba, Abdullahi Ibrahim; BESIII Collaboration
Phytolacca americana L. is of great interest as a traditional additive in various folk remedies in several countries, including Turkey. We aimed to determine the chemical profile (assisted by high-Performance liquid chromatography-electrospray ionization-tandem mass apectrometry (HPLC-ESI-MS/MS) experiments of three extracts obtained by different polarity solvents viz. ethyl acetate (to extract semipolar compounds), methanol and water (to extract highly polar metabolites) from P. americana leaves. Their anti-diabetic effects were investigated in vitro by assessing their inhibition toα-amylase and α-glucosidase. Assessment of the neuroprotective potential of the three extracts was carried out against acetyl-(AChE) and butyryl-(BChE) cholinesterase enzymes. HPLC-ESI-MS/MS experiments showed a total of 17 chromatographic peaks primarily classified to six flavonoids, two saponins, and six fatty acids. Antioxidant assays revealed remarkable activity for the ethyl acetate and methanol extracts. The BChE inhibition was considerably more significant (4.08 mg galantamine equivalent (GALAE)/g) for the ethyl acetate extract, whereas the methanol extract had good inhibitory efficacy for AChE (2.05 mg GALAE/g). Through network pharmacology, the compounds’ mechanism of action of targeted key gene in their associated diseases were identified. The hubb gene signal transducer and activator of transcription 3 (STAT3) and tumour necrosis factor (TNFα) where the P. americana compound’s site of action in inflammation bowel disease. The results offer possibilities for the prospective application of P. americana in metabolic regulation, blood glucose control, and as a source of bioactive compounds with cholinesterase enzyme inhibitory characteristics which could be of relevance in the cosmetic or pharmaceutical industry for combating melanogenesis. Communicated by Ramaswamy H. Sarma. © 2024 Informa UK Limited, trading as Taylor & Francis Group.
2024-01-01T00:00:00Z
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Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts
https://hdl.handle.net/20.500.12294/4067
Elucidating the chemical profile and biological studies of Verbascum diversifolium Hochst. extracts
Uba, Abdullahi Ibrahim; BESIII Collaboration
The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of V. diversifolium (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichloromethane (DCM), ethyl acetate (EtOAc), ethanol (EtOH), 70% EtOH, and water (aqueous) were prepared by maceration. The EtOH extract was predominated by the presence of rutin (4280.20 mu g g(-1)) and p-coumaric acid (3044.01 mu g g(-1)) while the highest accumulation of kaempferol-3-glucoside (1537.38 mu g g(-1)), caffeic acid (130.13 mu g g(-1)) and 4-hydroxy benzoic acid (465.93 mu g g(-1)) was recorded in the 70% EtOH, aqueous, and EtOAc extracts, respectively. The EtOH (46.86 mg TE/g) and 70% EtOH (46.33 mg TE/g) extracts displayed the highest DPPH radical scavenging result. Both these extracts, along with the aqueous one, exerted the highest ABTS radical scavenging result (73.03-73.56 mg TE/g). The EtOH and 70% EtOH extracts revealed the most potent anti-AChE (2.66 and 2.64 mg GALAE/g) and anti-glucosidase (1.07 and 1.09 mmol ACAE/g) activities. The aqueous extract was the most efficacious in inhibiting the proliferation of prostate cancer (DU-145) cells with an IC50 of 8.71 mu g/mL and a Selectivity Index of 3.7. In conclusion, this study appraised the use of V. diversifolium aerial parts as a potential therapeutic source for future development of phytopharmaceuticals that target specific oxidative stress-linked diseases including diabetes, cancer, cardiovascular disease, and Alzheimer's disease among others.
2024-01-01T00:00:00Z
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Antioxidant, enzyme inhibition, toxicity, and molecular docking analysis of Melaleuca cajuputi leaf extract and fractions
https://hdl.handle.net/20.500.12294/4033
Antioxidant, enzyme inhibition, toxicity, and molecular docking analysis of Melaleuca cajuputi leaf extract and fractions
Isah, Musa; Zengin, Gokhan; Wahab, Wan-Nor-Amilah Wan Abdul; Abdullah, Hasmah; Sul’ain, Mohd Dasuki; Uba, Abdullahi Ibrahim
Melaleuca cajuputi plant has piqued the interest of researchers due to its pharmacological properties. However, there is scarce information regarding its enzyme inhibitory effects and possible mechanism of action. Thus, this study aimed to investigate the antioxidant, enzyme inhibitory, and potential binding interactions of bioactive components in M. cajuputi extract and fractions with the target enzymes. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging (DPPH) assay was used for the antioxidant activity. The enzyme inhibition potential was assessed using tyrosinase and acetylcholinesterase (AChE) enzymes. M. cajuputi methanolic extract (MCME) demonstrated the most potent scavenging activity in comparison to Melaleuca fraction (MF1) and (MF2), as evidenced by IC50 values of 28.14 ± 0.96, 35.26 ± 0.64, and 80.90 ± 0.17 µg/mL, respectively. Similarly, the results of the enzyme inhibition assay showed that the MCME (IC50 65.81± 0.39 µg/mL) had the highest potency on tyrosinase enzyme compared to MF1 (IC50 131.3± 0.43 µg/mL) and MF2 (IC50 215.3 ± 0.48 µg/mL). However, MF2 (IC50 3.35 ± 0.37 µg/mL) showed a remarkable AChE enzyme inhibition than MCME and MF1. On the other hand, MCME possessed a low toxicity effect with an LC50 of 781 ± 0.07 µg/mL, whereas MF1 and MF2 were non-toxic. The 10-methylanthracene-9-carboxaldehyde, 2-isopropyl-10-methylphenanthrene, 2-tert butylanthracene, β-eudesmol, and α-eudesmol showed a strong binding propensity ranging from-5.1 to-6.2 and-6.4 to-7.6 kcal/mol towards tyrosinase and AChE respectively. The leaf extract of M. cajuputi could be considered a novel source of naturally occurring antioxidants and promising enzyme inhibitors. © 2024 Visagaa Publishing House.
2024-01-01T00:00:00Z