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dc.contributor.authorBaltacı, Oğuzhan Fatihen_US
dc.contributor.authorÇolakoğlu, Şeymaen_US
dc.contributor.authorGüllü Amuran, Gökçeen_US
dc.contributor.authorAydın, Neslihanen_US
dc.contributor.authorSargın, Mehmeten_US
dc.contributor.authorKarabay, Arzuen_US
dc.contributor.authorYılmaz, Temelen_US
dc.contributor.authorBerber, Ergülen_US
dc.date.accessioned2019-05-30T10:08:53Z
dc.date.available2019-05-30T10:08:53Z
dc.date.issued2018
dc.identifier.citationBaltaci, O. F., Colakoglu, S., Gullu Amuran, G., Aydin, N., Sargin, M., Karabay, A., . . . Berber, E. (2018). Exploring the role of miRNAs in the diagnosis of MODY3. Turkish Journal of Medical Sciences, 48(3), 620-+. doi:10.3906/sag-1711-98en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.urihttps://hdl.handle.net/20.500.12294/1436
dc.descriptionBerber, Ergül (Arel Author)en_US
dc.description.abstractBackground/aim: MODY3 associated with HNF1A is the most common form of MODY and is clinically misdiagnosed as type 1 diabetes due to similar clinical symptoms. This study aimed to analyze the role of HNF1A-regulated miRNAs as a biomarker in the diagnosis of MODY3. Materials and methods: MIN6 cells were transfected with the HNF1A cDNA expression vector for overexpression or with siRNA specific to HNF1A to silence its expression. The HNF1A-regulated miRNAs were determined by RNA-Seq of the total RNA extract. Expressions of the candidate miRNAs in blood samples of MODY3, type 1 diabetes, and type 2 diabetes patients and in healthy subjects were compared statistically by Mann-Whitney U tests.Results: This study revealed the presence of 238 known HNF1A-regulated miRNAs in MIN6 cells. miR-129-1-3p, miR-200b-3p, and miR-378a-5p were selected as candidate miRNAs. The expression level of miR-378a-5p significantly decreased in type 2 diabetes and MODY3 patients, while miR-200b-3p expression was significantly decreased only in MODY3 patients. Conclusion: Although further studies with larger numbers of patients are required, this study demonstrated that the expression levels of miR-200b-3p and miR-378a-5p decrease in MODY3 patients and suggests that miR-200b-3p is an especially strong candidate to use clinically for selecting suspected MODY3 patients.en_US
dc.language.isoengen_US
dc.publisherTUBITAKen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.identifier.doi10.3906/sag-1711-98en_US
dc.identifier.doi10.3906/sag-1711-98
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiomarkeren_US
dc.subjectDiabetesen_US
dc.subjectmiRNAen_US
dc.subjectMODY3en_US
dc.subjectmiR-200en_US
dc.subjectmiR-378en_US
dc.titleExploring the role of miRNAs in the diagnosis of MODY3en_US
dc.typearticleen_US
dc.departmentİstanbul Arel Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume48en_US
dc.identifier.issue3en_US
dc.identifier.startpage620en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/113S217


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