Hemşirelik Bölümü / Department of Nursing

The Comparison of DNA Extraction Techniques in Human Bone and Tooth Samples Exposed to High Heat

2022-10-03T19:05:38Z

The Comparison of DNA Extraction Techniques in Human Bone and Tooth Samples Exposed to High Heat Yükseloğlu, Emel Hülya; Dastan, Kadir; Yonar, Fatma Cavus; Rayimoglu, Gülten; Karatas, Ömer; Islek, Dilek Salkim; Doğan, Muzaffer Berna In the identification of forensic cases, it is critical to obtain information to identify the victim’s identity from burnt bone and tooth remains. When bone and tooth are burned, both their physical and chemical properties change significantly, which prevents anthropological evaluations and successful DNA profiling for identification purposes. Heat causes difficulties in the identification techniques in which bones and teeth are used, depending on the degree of heat exposure. For this reason, with the changes occurring in bone and tooth during combustion, it is necessary to evaluate the results of observation and analysis, and to be used the information obtained in a certain order. It is very difficult to extract DNA from the samples obtained from fire, explosion, motor vehicle and aircraft accidents and other traumatic events by traditional methods. The only biological material available to determine the typing and the identification of missing persons or unknown remains in different situations, such as mass fatality events, wars or socio-political events, is often only human remains. Effective DNA extraction procedures are critical steps in successful DNA analysis of skeletal remains. Due to variations in DNA and heterogeneity within the bone, unfortunately, the only reliable method for obtaining DNA from overly degraded specimens such as burnt bones and teeth in the literature, is still not available. In the study, present extraction methods are compared and as a result it was suggested that phenol-chloroform and Qiagen DNA Mini Kit could be used in routine with a newly developed modified procedure in order to increase efficiency and efficiency in forensic genetic identification.

Opinions About Death in People Receiving Dialysis

2022-10-03T18:44:52Z

Opinions About Death in People Receiving Dialysis Dönmez, Çiğdem Fulya; Yılmaz, M.; Helvacı, Ben Objective: People receiving dialysis have a high mortality rate due to life-threatening, chronic renal failure. These patients experience the fear of pain and suffering, loneliness and death in the haemodialysis unit. This research aimed at determining the perception of death in people receiving dialysis. Methods: A cross-sectional, descriptive research was conducted under the supervision of the Ministry of Health in public hospitals in the cities of Mersin, Izmir, Antalya, Erzurum, Samsun and Gaziantep. A total 240 patients were treated in the dialysis units of these hospitals. Participants were selected with stratified random sampling. For data collection, a patient information form was prepared by the researcher. Data from the study were analysed with Tukey Honest Significant Difference and one-way ANOVA, using an SPSS version 11.5 software package (Statistical Package for the Social Sciences Windows, IBM Corp., Armonk, NY). The statistical significance level was defined as p < 0.05. Results: People receiving dialysis were found to be in a mildly depressive emotional state and they had death anxiety. Death-related anxiety and depression were more common among the female study participants compared to the male participants. Single patients exhibited higher levels of death anxiety compared to married patients. Conclusion: We recommend a holistic and personalised care to allow people receiving dialysis to express their feelings and to overcome the death anxiety. Further research is needed to improve dignified person-centred care for people receiving dialysis.

The Effect of Pain Beliefs on Pain Levels in Cancer Patients

2022-10-03T19:05:31Z

The Effect of Pain Beliefs on Pain Levels in Cancer Patients Yıldırım, Dilek; Baykal, Dilek; Dedeoğlu Demir, Burcu Objective: The study was conducted to determine the effect of pain beliefs on pain levels in cancer patients. Methods: This descriptive and correlational study was completed with cancer patients (n=147) that treated in 3 different hospitals on oncology and chemotherapy units. Data were collected using the Socio-demographic Information Form, Visual Analogue Scale and Pain Beliefs Scale. Descriptive data were given as numbers, percentages and averages. Correlation test was used to determine the relationship between the pain level and the Pain Beliefs Scale. Results: The patients’ Pain Beliefs Scale scores of those who adapted to the disease were statistically significantly lower than those who could not (p=.028). Moreover, it was determined that those had stressful lives had statistically significantly higher Pain Belief Scale score that those who had not (p=0.025). It was found that the score that the patients got from the VAS pain intensity scale was 7.41±2.50, and it was high. The total score they got from the Pain Beliefs Scale was 4.23±.37. It was determined that there was a positive correlation between intensity of pain and organic beliefs in the subscale of the pain beliefs scale (r=.179, p˂.05). Conclusions: It was determined that organic pain beliefs of cancer patients have an impact on the level of pain. The pain beliefs of cancer patients were affected by adaptation to the disease and stressful life. Organic pain beliefs of cancer patients have an impact on the level of pain. Considering the relationship between individual’s pain belief and pain level in cancer pain management, planning treatment and nursing care is suggested.

Complementary and Alternative Medicine Use among Turkish Cancer Patients and the Influencing Factors

2022-10-03T19:03:14Z

Complementary and Alternative Medicine Use among Turkish Cancer Patients and the Influencing Factors Dedeoğlu Demir, Burcu; Erol, Özgül Background: The use of complementary and alternative medicine (CAM) therapies especially among cancer patients was quite frequent because of many reasons. Objective: The study was conducted in order to determine the use of CAM therapies among Turkish cancer patients and also determine the influencing factors. Methodology: This descriptive and cross-sectional study was performed with total 280 patients who received inpatient and outpatient treatment in an oncology clinic of a university hospital. Data were collected by using the Patient Characteristics Form and Complementary and Alternative Medicine Scale. p<0.05 was considered as statistically significant. Results: This study demonstrated that the patients who were women, and receiving outpatient care used energy approaches more often; patients who were single, and had metastatic disease used CAM approaches more often than the others. No significant difference was found between CAM use and education, occupation, performance score, diagnosis, and time of diagnosis. It was determined that 79.3% of the patients did not ask their physician about the use of CAM, and the knowledge about CAM use was taken from the newspaper/ television (36.1%), and friends (36.1%). Most frequent used approaches were nutritional (taking honey, 67.1%), cognitive behavioral (praying always, 41.1%), and biologic (drinking linden tea, 43.6%). The most common reason of CAM use was found as to strengthen the immune system (43.9%). Conclusion: The use of CAM therapies among Turkish cancer patients was quite frequent. Both health professionals and patients should be informed about the proper use of these approaches.