ArelPOTKAM

Production and characterization of non-leaching antimicrobial and hydrophilic polycaprolactone based nanofiber mats

Fri, 01 Jan 2021 00:00:00 GMT

Production and characterization of non-leaching antimicrobial and hydrophilic polycaprolactone based nanofiber mats Aynali, Figen; Balci, Huseyin; Doganci, Erdinc; Bulus, Erdi Novel antimicrobial and hydrophilic nanofiber biomaterial was fabricated with a combination of surface modification and nanotechnology, in this study. For this purpose, firstly poly(lactic acid) (PLA) bearing quaternary ammonium salt as an antimicrobial agent on its backbone in the rates of 5% by mole were successfully fabricated with a combination of ring-opening copolymerization and copper(I)-catalysed azide-–alkyne cycloaddition click reaction in a two-step reaction procedure. Then, this synthesized polymer was blended into polycaprolactone (PCL) matrix in three different proportions via electrospining technique. The prepared nanofiber mats were then characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and contact angle analysis. In addition, thermal properties of mats were examined through differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA) experiments. Its importance as an antimicrobial biomaterial was analyzed using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) which were types of Gram-positive and Gram-negative bacteria, respectively. As a result, it was observed that as the rate of antimicrobial polymer in the nanofiber material increased, its antimicrobial activity against both bacteria also increased. As a result of the highest blending ratio of antimicrobial PLA copolymer, the bacterial population amount decreased by 99.5% and 92% for S. aureus and E. coli, respectively, as compared to neat PCL nanofiber mat. Besides, thermal properties of PCL nanofiber mats did not changed negatively after the blending process, even char yield increased slightly with increasing rate of antimicrobial PLA content. Also, the results showed that the blending of PCL nanofiber mat with antimicrobial PLA having quaternary ammonium salts on its backbone maked it highly hydrophilic. © 2021 Elsevier Ltd

The in-vivo Assessment of Turkish Propolis and its Nano form on Testicular Damage Induced by Cisplatin

Fri, 01 Jan 2021 00:00:00 GMT

The in-vivo Assessment of Turkish Propolis and its Nano form on Testicular Damage Induced by Cisplatin Ismık, Deniz; Seven, Pnar Tatlı; Yedi, İsmail; Karakuş, Selcan; Mutlu, Seda İflazoğlu; Kaya, Şeyma Özer; Arkalı, Gözde; Tan, Ezgi; Şahin, Yeşim Müge; Kilislioğlu, Ayben Chemotherapeutic drugs, such as cisplatin (CP), which are associated with oxidative stress and apoptosis, may adversely affect the reproductive system. This study tests whether administration of propolis and nano-propolis (NP) can alleviate oxidative stress and apoptosis in rats with testicular damage induced by CP. In this study, polymeric nanoparticles including propolis were synthesized with a green sonication method and characterized using Fourier transform-infrared spectroscopy, Brunauer-Emmett-Teller, and wet scanning transmission electron microscopy techniques. In total, 56 rats were divided into the following seven groups: control, CP, propolis, NP-10, CP + propolis, CP + NP-10, and CP + NP-30. Propolis (100 mg/kg), NP-10 (10 mg/kg), and NP-30 (30 mg/kg) treatments were administered by gavage daily for 21 d, and CP (3 mg/kg) was administered intraperitoneally in a single dose. After the experiment, oxidative stress parameters, namely, malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), and catalase (CAT), and apoptotic pathways including B cell leukemia/lymphoma-2 protein (Bcl-2) and Bcl-2-associated X protein (Bax) were measured in testicular tissues. Furthermore, sperm quality and weights of the testis, epididymis, right cauda epididymis, seminal vesicles and prostate were evaluated. Propolis and NP (especially NP-30) were able to preserve oxidative balance (decreased MDA levels and increased GSH, CAT, and GPx activities) and activate apoptotic pathways (decreased Bax and increased Bcl-2) in the testes of CP-treated rats. Sperm motility in the control, CP, and CP + NP-30 groups were 60%, 48.75%, and 78%, respectively (P < 0.001). Especially, NP-30 application completely corrected the deterioration in sperm features induced by CP. The results show that propolis and NP treatments mitigated the side effects of CP on spermatogenic activity, antioxidant situation, and apoptosis in rats.

Dual-Drug Delivery of Ag-Chitosan Nanoparticles and Phenytoin Via Core-Shell PVA/PCL Electrospun Nanofibers

Fri, 01 Jan 2021 00:00:00 GMT

Dual-Drug Delivery of Ag-Chitosan Nanoparticles and Phenytoin Via Core-Shell PVA/PCL Electrospun Nanofibers Şahin, Yeşim Müge; Hüseyin, Mohamed Ahmed Mohamady; Güler, Ece; Rayaman, Erkan; Cam, Muhammet Emin; Şahin, Ali; Grinholc, Mariusz; Mansuroğlu, Demet Sezgin; Gündüz, Oğuzhan; Muhammed, Memun; El-Sherbiny, İbrahim M.; Meghed, Mosaad Dual-drug delivery systems were constructed through coaxial techniques, which were convenient for the model drugs used the present work. This study aimed to fabricate core-shell electrospun nanofibrous membranes displaying simultaneous cell proliferation and antibacterial activity. For that purpose, phenytoin (Ph), a well-known proliferative agent, was loaded into a polycaprolactone (PCL) shell membrane, and as-prepared silver-chitosan nanoparticles (Ag-CS NPs), as biocidal agents, were embedded in a polyvinyl alcohol (PVA) core layer. The morphology, chemical composition, mechanical and thermal properties of the nanofibrous membranes were characterized by FESEM/STEM, FTIR and DSC. The coaxial PVA-Ag CS NPs/PCL-Ph nanofibers (NFs) showed more controlled Ph release than PVA/PCL-Ph NFs. There was notable improvement in the morphology, thermal, mechanical, antibacterial properties and cytobiocompatibility of the fibers upon incorporation of Ph and Ag-CS NPs. The proposed core-shell PVA/PCL NFs represent promising scaffolds for tissue regeneration and wound healing by the effective dual delivery of phenytoin and Ag-CS NPs.

Swelling behaviour, rheological property and drug release profile of the anti-inflammatory drug metamizole sodium from xanthan gum-ZnO nanoparticles

Fri, 01 Jan 2021 00:00:00 GMT

Swelling behaviour, rheological property and drug release profile of the anti-inflammatory drug metamizole sodium from xanthan gum-ZnO nanoparticles Karakuş, Selcan; Tan, Ezgi; Ilgar, Merve; Şahin, Yeşim Müge; Mansuroğlu Sezgin, Demet; İsmik, Deniz; Somroo, Razium Ali; Kilislioğlu, Ayben In this study, our aim was to determine the effect of the polymer matrix on the drug delivery system; active-drug-loaded xanthan-gum (XaG)-based ZnO nanoparticles were synthesized under the ultrasonic irritation method. We characterized the structure using UV-Vis, SEM-EDX, FTIR and XRD techniques. The morphology of the nanoparticles has been carried out using SEM, and the results revealed that hexagonal disc structures were prepared with a size below 100 nm. The consideration of theoretical error analysis in estimating kinetic model parameters for simulating the drug release profile was incurred, to find a general model applicable to multimechanistic release. Five error analysis methods for identifying the kinetic model parameters have been calculated and their comparison based on goodness of fit. Results showed that drug-loaded XaG/ZnO nanoparticles released the drug with the Higuchi model (R-2 = 0.97-0.99). We compared the viscosities of nanostructures obtained by using different synthesis methods such as magnetic stirring and ultrasonic irradiation at different times (10-20 min) to illuminate the rheological structure. Comparing Krigbaum and Wall parameters (Delta b) for all samples, XaG/ZnO nanoparticles synthesized by ultrasonic irritation method proved to be an optimal miscible formulation. From the results, XaG/ZnO nanoparticles can potentially be recommended as a reliable nanocarrier with the release of metamizole sodium (MS) drug.