Dahili Tıp Bilimleri Bölümü Makale Koleksiyonu

Comparison of microRNA expression levels in patients with schizophrenia before and after electroconvulsive therapy

Mon, 01 Jan 2024 00:00:00 GMT

Comparison of microRNA expression levels in patients with schizophrenia before and after electroconvulsive therapy Saglam, Nazife Gamze Usta; Duz, Mehmet Bugrahan; Yilmaz, Seda Salman; Ozen, Mustafa; Balcioglu, Ibrahim ObjectiveExploring the role of microRNAs in the antipsychotic efficacy of electroconvulsive therapy (ECT) will contribute to understanding the underlying mechanism through which ECT exerts its therapeutic effects. The primary objective of this study was to identify microRNA alterations before and after ECT in patients with schizophrenia.MethodsWe compared microarray-based microRNA profiles in peripheral blood from eight patients with schizophrenia before and after ECT and eight healthy controls. Then, we aimed to validate selected differentially expressed microRNAs in 30 patients with schizophrenia following a course of ECT, alongside 30 healthy controls by using quantitative reverse-transcription PCR.ResultsMicroarray-based expression profiling revealed alterations in 681 microRNAs when comparing pre- and post-ECT samples. Subsequent quantitative reverse-transcription PCR analysis of the selected microRNAs (miR-20a-5p and miR-598) did not reveal any statistical differences between pre- and post-ECT samples nor between pre-ECT samples and those of healthy controls.ConclusionAs neuroepigenetic studies on ECT are still in their infancy, the results reported in this study are best interpreted as exploratory outcomes. Additional studies are required to explore the potential epigenetic mechanisms underlying the therapeutic efficacy of ECT.

The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line

Mon, 01 Jan 2024 00:00:00 GMT

The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line Tanriverdi, Gamze; Kaleci, Belisa; Yavuz, Furkan; Sahin, Hakan; Purelku, Merjem; Yazici, Zeliha; Kokturk, Sibel Glioblastoma tumors are the most aggressive primary brain tumors that develop resistance to temozolomide (TMZ). Eribulin (ERB) exhibits a unique mechanism of action by inhibiting microtubule dynamics during the G2/M cell cycle phase. We utilized the T98G human glioma cell line to investigate the effects of ERB and TMZ, both individually and in combination. The experimental groups were established as follows: control, E5 (5 nM ERB), T0.75 (0.75 mM TMZ), T1 (1.0 mM TMZ), and combination groups (E5+T0.75 and E5+T1). All groups showed a significant decrease in cell proliferation. Apoptotic markers revealed a time-dependent increase in annexin-V expression, across all treatment groups at the 48-hour time point. Caspase-3, exhibited an increase in the combination treatment groups at the 48-hour mark. Transmission electron microscopy (TEM) revealed normal ultrastructural features in the glioma cells of the control group. However, treatments induced ultrastructural changes within the spheroid glioblastoma model, particularly in the combination groups. These changes included a dose-dependent increase in autophagic vacuoles and apoptotic morphology of the cells. In conclusion, the similarity in the mechanism of action between ERB and TMZ suggests the potential for synergistic effects when combined. Our results highlight that this combination induced severe damage and autophagy in glioma spheroids after 48 hours.

The Relationship between Frontal QRS-T Angle and Vitamin D Deficiency

Mon, 01 Jan 2024 00:00:00 GMT

The Relationship between Frontal QRS-T Angle and Vitamin D Deficiency Demir, Fulya Avci; Bingol, Gulsum; Ersoy, Ibrahim; Arslan, Akif; Ersoy, Pinar; Demir, Meltem; Unlu, Serkan Background and Objectives: A deficiency in serum 25-hydroxyvitamin D levels is associated with a number of cardiovascular situations, such as high blood pressure, heart failure, atherosclerotic heart disease, and peripheral artery disease. The frontal QRS-T angle has recently been proposed as a marker of ventricular repolarization. A wider frontal QRS-T angle has been positively correlated with adverse cardiac events. The objective of our study was to examine the association between serum 25-hydroxyvitamin D level and the frontal QRS-T angle. Materials and Methods: A total of 173 consecutive patients aged 18-60 years undergoing routine cardiology check-up evaluation, and not receiving concurrent vitamin D treatment were included in the study. Patients were classified in three groups, depending on their vitamin D levels, and categorized as follows: Group 1-deficient (<20 ng/mL), Group 2-insufficient (20-29 ng/mL), or Group 3-optimal (>= 30 ng/mL). The frontal QRS-T angle was determined using the automated reports generated by the electrocardiography machine. Results: The average age of participants was 45.8 (+/- 12.2) years, and 55.5% of participants were female (p < 0.001). Individuals with low vitamin D concentrations exhibited a wider frontal QRS-T angle. It was determined that vitamin D level is an independent predictive factor for the frontal QRS-T angle. Conclusions: As the levels of 25-hydroxyvitamin D decrease, repolarization time assessed by frontal QRS-T angle is widened. Our findings indicate that lower concentrations of vitamin D may increase the susceptibility to ventricular arrhythmia.

A Visceral Leishmaniasis Case from the Black Sea Region

Mon, 01 Jan 2024 00:00:00 GMT

A Visceral Leishmaniasis Case from the Black Sea Region Onec, Birguel; Ozturk, Cihadiye Elif; Yazici, Ayten Karadeniz Bölgesinden Bir Visseral Leishmaniasis Olgusu: Deri Lezyonları ve Kemik İliğinde Leishmania donovani Amastigotları.