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Characterization of a new acidic NAD(+)-dependent formate dehydrogenase from thermophilic fungus Chaetomium thermophilum

Özgün, Gülşah; Karagüler, Nevin Gül; Turunen, Ossi; Turner, Nicholas J.; Binay, Barış (ELSEVIER SCIENCE BV, 2015)

NAD(+)-dependent formate dehydrogenase (FDH) enzyme catalyses the NAD(P)(+)-dependent interconversion of formate anion to carbon dioxide coupled with the conversion of NAD(P)(+) to NAD(P)H. NADH is widely used as cofactor ...

Highly stable and reusable immobilized formate dehydrogenases: Promising biocatalysts for in situ regeneration of NADH

Binay, Barış; Alagöz, Dilek; Yıldırım, Deniz; Çelik, Ayhan; Tükel, S. Seyhan (2016)

This study aimed to prepare robust immobilized formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. ...

Improving of stability of formate dehydrogenase from candida methylica by immobilization onto eupergit C 250 L

Alagöz, D.; Binay, Barış; Yıldırım, D.; Çelik, A.; Tükel, S. S. (Pleiades Publishing, 2016)

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Semi-rational design of geobacillus stearothermophilus l-lactate dehydrogenase to access various chiral ?-hydroxy acids

Aslan, Aşkın Sevinç; Birmingham, William R.; Karagüler, Nevin Gül; Turner, Nicholas J.; Binay, Barış (Springer, 2016)

Chiral ?-hydroxy acids (AHAs) are rapidly becoming important synthetic building blocks, in particular for the production of pharmaceuticals and other fine chemicals. Chiral compounds of a variety of functionalities are now ...

Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment

Albuquerque, Tiago L.de; Rueda, Nazzoly; dos Santos, Jose C.S.; Barbosa, Oveimar; Ortiz, Claudia; Binay, Barış; Özdemir, Ece; Gonçalves, Luciana R.B.; Fernandez-Lafuente, Roberto (Elsevier, 2016)

Octyl-agarose is a support that permits the one step immobilization, stabilization and purification of lipases. However, the enzyme may be released from the support under drastic conditions. This paper describes a new ...

Aktif Bölge Mimarisinin Anlaşılması Temelinde, NAD + Bağımlı Mutant Candida methylica Format Dehidrogenaz Enziminin Spesifik Aktivitesinin Arttırılması

Binay, Barış; Torun, Soner (TÜBİTAK KBAG Proje, 2023)

Format dehidrogenaz enzimi (FDH), endüstriyel biyokatalizörler arasında son yıllarda en çok dikkat çeken enzimlerden biridir. Özellikle NAD(P)+ bağımlı FDH, gerçekleştirdiği reaksiyonun düşük redoks potansiyeli nedeniyle ...